Anti-allergic function of -Tocopherol is mediated by suppression of PI3K-PKB activity in mast cells in mouse model of allergic rhinitis
Main Article Content
Keywords
α-TCP, Allergic rhinitis, PI3K-PKB, Animal model, OVA
Abstract
Background: Alpha-Tocopherol (-TCP), one major form of vitamin E, has been known as a treatment for airway allergic inflammation. However, the role and mechanism of -TCP in treating allergic rhinitis remains unclear.
Objective: In this study, we examined the inhibitory function of -TCP in a mouse model of allergic rhinitis.
Methods: Allergic phenotype was examined by hematoxylin and eosin staining. Total IgE, OVAspecific IgE, OVA-specific IgG1 and OVA-specific IgG2a levels were examined by ELISA. mRNA expression was measured by qPCR, protein levels were examined by Western Blot.
Results: Histological analysis of the nasal membranes revealed that there was a significant reduction in inflammatory cells appearance in cross-sections in alpha-TCP treatment of Ovalbumin (OVA)-sensitized mice compared to OVA-sensitized animals. In addition, eosinophils were significantly reduced in nasal mucosa of alpha-TCP treatment of OVA-sensitized mice compared to the OVA group. Lower total IgE, OVA-specific IgE, OVA-specific IgG1 and OVA-specific IgG2a levels were found in alpha-TCP treatment of OVA-sensitized mice compared to the OVA group. Furthermore, we found that the subepithelial distribution of tryptase positive mast cells was reduced in the alpha-TCP treatment of OVA-sensitized mice. More importantly, the PI3K-PKB pathway was suppressed by -TCP in mast cells.
Conclusions: Our results demonstrated that -TCP-mediated suppression of PI3K-PKB activity in mast cells is a potential mechanism of anti-allergic function of -TCP.
References
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