Dust mite-derived Enterobacterial fimbriae H protein enforces the allergen specific immunotherapy in asthma mice

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X. Yang
State Key Laboratory of Respiratory Disease for Allergy at Shenzhen University, Shenzhen Key Laboratory of Allergy & Immunology, Shenzhen University School of Medicine, Shenzhen, 518060, China; Shenzhen Luohu Maternity and Child Healthcare Hospital, #2013 Taibai Road, Shenzhen, 518019, China.

H. Wang
State Key Laboratory of Respiratory Disease for Allergy at Shenzhen University, Shenzhen Key Laboratory of Allergy & Immunology, Shenzhen University School of Medicine, Shenzhen, 518060, China.

D. Zhao
Shenzhen Luohu Center for Chronic Disease Control, #11 Jinhu Road, Shenzhen, 518023, China.

J. Wang
State Key Laboratory of Respiratory Disease for Allergy at Shenzhen University, Shenzhen Key Laboratory of Allergy & Immunology, Shenzhen University School of Medicine, Shenzhen, 518060, China; Allergy & Inflammation Research Unit, Department of Respiratory Medicine, Chengdu Institute of Respiratory Health, the Third People’s Hospital of Chengdu, Chengdu, 610031, Sichuan, China.

X. Liu
State Key Laboratory of Respiratory Disease for Allergy at Shenzhen University, Shenzhen Key Laboratory of Allergy & Immunology, Shenzhen University School of Medicine, Shenzhen, 518060, China.

X. Yuan
State Key Laboratory of Respiratory Disease for Allergy at Shenzhen University, Shenzhen Key Laboratory of Allergy & Immunology, Shenzhen University School of Medicine, Shenzhen, 518060, China.

M. Zhang
State Key Laboratory of Respiratory Disease for Allergy at Shenzhen University, Shenzhen Key Laboratory of Allergy & Immunology, Shenzhen University School of Medicine, Shenzhen, 518060, China.

G. Li
State Key Laboratory of Respiratory Disease for Allergy at Shenzhen University, Shenzhen Key Laboratory of Allergy & Immunology, Shenzhen University School of Medicine, Shenzhen, 518060, China; Allergy & Inflammation Research Unit, Department of Respiratory Medicine, Chengdu Institute of Respiratory Health, the Third People’s Hospital of Chengdu, Chengdu, 610031, Sichuan, China.

P. Ran
Guangzhou Institute of Respiratory Diseases, State Key Lab of Respiratory Diseases, The First Affiliated Hospital, Guangzhou Medical University, Guangzhou, Guangdong, 511436, China.

P. Yang
State Key Laboratory of Respiratory Disease for Allergy at Shenzhen University, Shenzhen Key Laboratory of Allergy & Immunology, Shenzhen University School of Medicine, Shenzhen, 518060, China.

Z. Liu
State Key Laboratory of Respiratory Disease for Allergy at Shenzhen University, Shenzhen Key Laboratory of Allergy & Immunology, Shenzhen University School of Medicine, Shenzhen, 518060, China; Guangzhou Institute of Respiratory Diseases, State Key Lab of Respiratory Diseases, The First Affiliated Hospital, Guangzhou Medical University, Guangzhou, Guangdong, 511436, China.

Keywords

Allergen specific immunotherapy, House dust mite, Enterobacterial fimbriae H protein, Tregs, Adjuvant effect

Abstract

Background: The mite alimentary canal contains plenty of microbiota. It is accepted that some of the microbial products function as adjuvants to speed up immune responses.


Objectives: We identified five bacterial proteins from dust mite, and Enterobacterial fimbriae H (FimH) was one of them. This study aims to test a hypothesis that the FimH protein enforces immunotherapy in asthmatic mice.


Methods: Asthmatic mice were treated by allergen specific immunotherapy (ASIT) with rDer f1/f2 or rDer f1/f2 plus FimH. Changes in inflammatory cell infiltration, airway hyperactivity, frequency of Tregs, splenic CD4+ IFN-y+ cells, and serum levels of TGF-, IL-10, IL-13 and IL-17A of asthmatic mice were checked.


Results: ASIT with rDer f1/f2 plus FimH reduced inflammatory cell infiltration, airway hyperreactivity (AHR), and levels of IgE and IgG1 compared to ASIT with rDer f1/f2 alone, but the levels of IgG2a increased. Asthmatic mice that underwent ASIT with rDer f1/f2 plus FimH showed increased frequency of Tregs, splenic CD4+ IFN-y+ cells, serum levels of TGF- and IL10; and deceased splenic CD4+ IL-4+ cells, and serum levels of IL-13 and IL-17A. In vitro study showed FimH triggered IL-10 expression in a concentration-dependent manner and facilitated the differentiation of Tregs.


Conclusion: Used as an adjuvant, FimH enforces the effect of ASIT in asthmatic mice via augmenting Tregs.

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Dec 15, 2020

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Yang, X., Wang, H., Zhao, D., Wang, J., Liu, X., Yuan, X., Zhang, M., Li, G., Ran, P., Yang, P., & Liu, Z. (2020). Dust mite-derived Enterobacterial fimbriae H protein enforces the allergen specific immunotherapy in asthma mice. Allergologia Et Immunopathologia, 48(6), 654-665. https://all-imm.com/index.php/aei/article/view/133
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Vol. 48 No. 6 (2020): Allergologia et Immunopathologia
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Original Articles
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Yang, X., Wang, H., Zhao, D., Wang, J., Liu, X., Yuan, X., Zhang, M., Li, G., Ran, P., Yang, P., & Liu, Z. (2020). Dust mite-derived Enterobacterial fimbriae H protein enforces the allergen specific immunotherapy in asthma mice. Allergologia Et Immunopathologia, 48(6), 654-665. https://all-imm.com/index.php/aei/article/view/133