Antibodies to Der p 1 and Der p 2 in allergic patients

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Renata Harumi Cruz
Leandro Hideki Ynoue
Carolina Sanchez Aranda
Dirceu Solé
Antonio Condino Neto


allergen, allergy, Dermatophagoides pteronyssinus, antibodies


Introduction and objectives: Atopic individuals are characterized by increased IgE production and Th2 response if exposed to certain antigens. It is known that the mother transfers antimite antibodies to the fetus and newborn, IgG thru the placenta, and IgA thru breastfeeding, but it is not clear whether there is a protective mechanism mediated by them concerning the development of future allergies. This study aimed to compare the levels of IgA, IgG, and IgE antibodies specific to Der p 1 and Der p 2 between atopic and healthy individuals.
Methods: Serum samples of 98 patients and 44 healthy controls were subjected to quantification for specific IgE, IgG, and IgA antibodies against Der p 1 and Der p 2 by ImmunoCap® and ELISA, and subjected to statistical analysis as indicated.
Results: Atopic patients had higher serum levels of IgE, IgG, and IgA specific to Der p 1 and Der p 2. The correlation was more robust between IgE and IgG antibodies.
Conclusions: Allergic patients produce higher levels of antibodies against Der p 1 and Der p 2 compared with healthy individuals. The mechanisms involved still require detailed studies.

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1. Tan H-TT, Sugita K, Akdis CA. Novel biologicals fot the treatment of allergic diseases and asthma. Curr Allergy Asthma Rep. 2016;16(70):1–14.

2. Hawrylowicz CM, Garra AO. Potencial role of interleukin-10 – Secreting regulatory T cells in allergy and asthma. Nature Rev. 2005;5:271–83.

3. Rutkowski K, Sowa P, Talipska JR, Sulkowski S, Rutkowski R. Allergic diseases: The price of civilisational progress. Derm Alergol. 2014;2:77–83.

4. London Jr R, Tharakan A, Ramanathan Jr M. The role of innate immunity and aeroallergens in chronic rhinosinusites. Adv Otorhinolaryngol Basel (Karger). 2016;79:66–77.https://doi. org/10.1159/000445132

5. Yazdanbakhsh M, Kremsner PG, Ree van R. Allergy, parasites and the hygiene hypothesis. Science. 2002;296:490–4. https://

6. Aranda CS, Cocco RR, Pierotti FF, Mallozi MC, Franco JM, Porto A, et al. Increased sensitization to several allergens over a 12-year period in Brazilian children. Pediatr Allergy Immunol. 2018;29(3):321–4.

7. Platts-Mills TAE, de Weck AL. Dust mite allergens and asthma–A worldwide problem. J Allergy Clin Immunol. 1989;83:416– 27.

8. Criado RFJ, Wandalsen NF. Fatores ambientais em alergia. Alergia e Imunologia na Infância e na Adolescência. Atheneu. São Paulo. 2001:13–22.

9. Serravalle K, Medeiros M Jr. Ácaros da poeira domiciliar na cidade de Salvador-BA. Rev Bras Alerg Imunopatol. 1999;22:19–24.

10. Platts-Mills TAE, Chapman MD. Dust mites: Immunology, allergic disease, and environmental control. J Allergy Clin Immunol. 1987;80:755–77.

11. Tsaia JJ, Shenb HD, Chuac KY. Purification of group 2 dermato-phagoides pteronyssinus allergen prevalence of its specific IgE in asthmatics. Int Arch Allergy Immunol. 2000;121:205–

12. Marie-Eve D, Herman J, Campizi V, Galleni M, Jacquet A, Chevigné A. Orchestration of an uncommon maturation cascade of the house dust mite protease allergen quartet. Front Immunol Immunotherap Vaccines. 2014;5(138):1–6. https://

13. Valk van der JPM, Jong de NW, Wijk van RG. Review on immunotherapy in airway allergen-sensitised patients. Neth J Med. 2015;73(6):263–96.

14. Till SJ, Francis JN, Nouri-Aria K, Path FRC, Durham SR. Molecular mechanisms in allergy and clinical immunology. J Allergy Clin Immunol. 2004;113(6):1025–34.https://doi. org/10.1016/j.jaci.2004.03.024

15. Shamji MH, Durham SR. Machanisms of allergen immunotherapy for inhaled allergens and predictive biomarkers. J Allergy Clin Immunol. 2017;140(6):1485–98. jaci.2017.10.010

16. Uphan JW. The role of dendritic cells in immune regulation and allergic airway inflammation. Respirology. 2003;8:140–48.

17. Chauveau A, Dalphin ML, Mauny F, Kaulek V, Schmausser-Hechfellner E, Renz H, et al. Skin prick tests and specific IgE in 10-year-old children: Agreement and association with allergic diseases. Allergy. 2017;72(9):1365–1373. https://doi. org/10.1111/all.13148

18. Haahtela T, Burbach GJ, Bachert C, Bindslev-Jensen C, Bonini S, Bousquet J, et al. Clinical relevance is associated with allergen-specific wheal size in skin prick testing. Clin Exp Allergy. 2014;44:407–416.

19. Boechat JL, França AT. Atopic march. Revista Brasileira de Alergia e Imunopatologia. 2008;31(4):139–45.

20. Roche N, Chinet TC, Huchon GJ. Allergic and nonallergic interactions between house dust mite allergens and airway mucosa. Eur Resp J. 1997;10:719–26.

21. Rönmark EP, Ekerljung L, Mincheva R, Sjölander S, Hagstads Wennergren G, Rönmark E, et al. Different risk factor patterns for adult asthma, rhinitis and eczema: Results from West Sweden Asthma Study. Clin Translat Allergy. 2016;6(28):1–10.

22. Minami T, Fukutomi Y, Lidholm J, Yasueda H, Saito A, Sekiya K, et al. IgE abs to Der p 1 and Der p 2 as diagnostic markers of house dust mite allergy as defined by a bronchoprovocation test. Allergil Int. 2015;64:90–95. alit.2014.08.004

23. Saunders SP, Ma EGM, Aranda CJ, Curotto de Lafaille MA. Non-classical B cell memory of allergic IgE responses. Front Immunol. 2019;10:715. 00715

24. Hales BJ, Martin AC, Pearce LJ, Laing IA, Hayden CM, Goldblatt J, et al. IgE and IgG anti-house dust mite specificities in allergic disease. J Allergy Clin Immunol. 2006;118:361–7.

25. Smart JM, Kemp AS. Increased Th1 and Th2 allergen-in-duced cytokine response in children with atopic disease. Clin Exp Allergy. 2002;32:796–802. https://doi. org/10.1046/j.1365-2222.2002.01391.x

26. Akdis CA, Akdis M. Mechanisms of immune tolerance to allergens: Role of IL-10 and T regs. J Clin Invest. 2014;124(11):4678– 80.

27. Kim JH, Yoon MG, Seo DH, Kim BS, Ban GY, Ye YM, et al. Detections of allergen-specific antibodies from nasal secretion of allergic rhinitis patients. 2016;8(4):329–37. https://doi. org/10.4168/aair.2016.8.4.329

28. Suzuki K, Fagarsan S. Diverse regulatory pathways for IgA syn-tesis in the gut. Mucosal Immunol Nature. 2009;6(2):468–471.

29. Schwarz A, Panetta V, Capella A, Hofmaier S, Hatzler L, Rohrbach A, et al. IgG and IgG4 to 91 allergenic molecules in early childhood by route of exposure and current and future IgE sensitization: Results from the Multicentre Allergy Study birth cohort. J Allergy Clin Immunol. 2016;138(5):1426–33.