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Management of hereditary angioedema type I and homozygous MTHFR mutation during pregnancy

Amanda Ribeiro Batllea*, Ana Paula Possar do Carmoa, Nirelcio Galaob, Anete S Grumachc

aMedical student, Faculdade de Medicina do ABC, Santo Andre, SP, Brazil

bClinics in Gynecology and Obstetrics, Londrina, PR, Brazil

cClinical Immunology, Reference Center for Rare Diseases, Faculdade de Medicina do ABC, Santo Andre, SP, Brazil

Abstract

Hereditary angioedema (HAE) is an autosomal dominant disease, characterized by edema attacks resulting from quantitative and/or functional deficiency of the C1 inhibitor (C1-INH), which acts in controlling the complement, coagulation, fibrinolysis, and contact systems. The exacerbation of these systems results in decreased circulating levels of kallikrein and conversion of bradykinin. In addition, thrombophilia is related to the deficiency of methylenetetrahydrofolate reductase (MTHFR) enzyme, causing an increase in homocysteine, accumulation of atheromatous plaques, and arterial and venous thrombosis. The association of these conditions in related systems brings greater clinical risks to the patient. We report a female patient, aged 23 years, with HAE and homozygous MTHFR mutation, G2A1, carrier of HAE with crises since early childhood. The first pregnancy terminated with abortion due to gestational sac detachment. In the second pregnancy, at 5.1 weeks, she had bleeding and partial detachment of gestational sac. Thrombophilia tests confirmed homozygosity for the MTHFR enzyme. At the beginning of gestation, she had attacks of angioedema treated with fresh plasma, and at one occasion, she received treatment with a plasma-derived C1-INH esterase. During breastfeeding, she received prophylaxis with plasma-derived C1-INHdp. The course of HAE during pregnancy worsened. There are studies that discuss the occurrence of abortion due to attacks of angioedema. The patient’s disease was associated with a homozygous MTHFR mutation, which probably caused the miscarriage. The control of both clinical situations is important for the success of pregnancy, so a combined action plan between obstetricians and HAE treatment specialists is essential.

Key words: hereditary angioedema, MTHFR mutation, pregnancy, thrombosis, thrombophilia

*Corresponding author: Anete Sevciovic Grumach, MD, PhD, CEPES, Research Center, Av Lauro Gomes, 2000 Santo Andre, 09060.870 SP, Brazil. Email address: asgrumach@gmail.com

Received 6 November 2020; Accepted 11 February 2021; Available online 1 July 2021

DOI: 10.15586/aei.v49i4.57

Copyright: Amanda Ribeiro Batlle, et al.
License: This open access article is licensed under Creative Commons Attribution 4.0 International (CC BY 4.0). http://creativecommons.org/licenses/by/4.0/

Introduction

Hereditary angioedema (HAE) is a rare disease with an approximate prevalence of 1:50,000.1 Inheritance is autosomal dominant and there is a quantitative (type 1) and/or functional (type 2) impairment of the C1 esterase inhibitor (C1-INH). C1-INH acts in several pathways: complement, fibrinolytic, coagulation, and contact systems. HAE generally manifests in the first decade of life and is characterized by abdominal symptoms and episodes of angioedema on the face, genitalia, extremities, urinary tract, and upper airways.1 Another form of HAE with normal C1-INH was described and had similar clinical features to HAE, mainly affecting women after puberty. However, it was related to high levels of estrogen.2

Pregnancy influences the course of HAE in different ways: it may aggravate or have no effect on edematous attacks. We previously showed increasing number of attacks during the second trimester3 of pregnancy, while others reported worsening at the third trimester.4,5 During this period, high levels of progesterone reach a plateau, and simultaneously an increase in the concentration of estrogen and placental prolactogenic hormones is visible, resulting in a greater frequency of edema attacks.6 The recommended treatment for acute episodes during pregnancy is the infusion of C1-INH concentrate; however, when this concentrate is not available, fresh frozen plasma is administered.7 Long-term prophylaxis should be considered according to four variables analyzed: frequency and severity of attacks, impact on the quality of life, and availability of drugs for therapy. On account of this if long-term prophylaxis is indicated, it should be introduced as soon as possible.

During gestation, thrombophilia becomes a relevant factor due to the tendency for hypercoagulability related to the elevation of procoagulant factors and, consequently, the possibility of obstetric complications.8 In addition, thrombophilia could be related to the deficiency of the methylenetetrahydrofolate reductase (MTHFR) enzyme responsible for the conversion of 5,1-methylenetetrahydrofolate to 5-methyltetrahydrofolate, which assists in the methylation of homocysteine into methionine. MTHFR deficiency interferes with this conversion, causes plasma homocysteine accumulation, and predisposes to atheroma plaques and arterial and venous thromboses.9

C1-INH also controls the activation of Factor XII. Thus, the association of HAE–C1-INH and thrombophilia can result in vascular complications during pregnancy. A recent report on HAE pregnancy associated with heterozygous MTHFR mutation has shown the enhancement of attacks and seven abortions preceding a successful pregnancy.8 We report management of a patient during gestation period with HAE type I and the homozygous MTHFR mutation.

Case Report

A 23-year-old female with HAE type 1 reported recurrent episodes of angioedema affecting the face and respiratory difficulty since infancy. The first miscarriage due to gestational sac detachment lead to the investigation of thrombophilia. During the second gestation, at 5.1 weeks, there was bleeding and partial detachment of the gestational sac with no harm to the embryo. At this time, oral progesterone (200 mg/day) was introduced. The obstetrician requested evaluation for thrombophilia and a homozygous mutation of MTHFR gene was identified. The following drugs were introduced: enoxaparin (40 mg/day, subcutaneously, after the 11th week of gestation), aspirin (100 mg/day up to 32 weeks of gestation), folic acid (5 mg/day), calcium carbonate (500 mg), and Vitamin D. Moreover, the patient used progestins for an 8-week period starting at week 9 of pregnancy, and again from week 26 to week 36 (Figure 1). No prophylactic therapy for HAE was used during the pregnancy. Attacks occurred at 9th, 19th, and 29th week of pregnancy and were treated with fresh frozen plasma in one episode due to unavailability of C1-INH concentrate at that time. Mild attacks were not treated but followed up closely. The pregnancy evolved without further complication and with an adequate progression of uterine height. A cesarean section was performed at 38.2 weeks of gestation after prophylaxis with plasma-derived esterase C1-INH (pdC1-INH; 1000 IU). The patient was discharged after 48 h and prescribed noxaparin (40 mg) until the 15th day of puerperium. The attacks restarted at every 5 days during puerperium and breastfeeding. Then, this time, prophylaxis with plasma-derived esterase C1-INH was introduced at every 7 days. After this, the child’s development was within normal limits. The patient’s sister also had HAE and heterozygous polymorphism for MTHFR (A1298C); she got pregnant just after the patient, with frequent HAE attacks but no other complication: her tests did not show thrombophilia and heparin was not prescribed. Furthermore, she had access to plasma-derived C1-INH that was prescribed for prophylaxis during the entire gestation. No family history of thrombosis was found. Their parents were asymptomatic and had never been tested for C1-INH deficiency.

Figure 1 Treatment medications for the patient and uterine height over time.

DISCUSSION

Appropriate monitoring is required during pregnancy of women with HAE, especially because of its unpredictable course and, as in the discussed case, aggravated by the previous report of abortion and gestational sac detachment, conducting to investigation of thrombophilia. Angioedema attacks affect the same sites in both pregnant and non-pregnant women, with the exception of abdominal attacks, which are prevalent during this period. Differential diagnosis of HAE attacks and uterine contractions could be difficult in this situation. Abdominal symptoms were not relevant to our patient; nonetheless, three severe attacks with involvement of upper airway were reported. No attacks occurred at the third trimester but they recurred after delivery. Our patient delivered through a cesarean section, even though it was not a mandatory procedure for HAE patients. In fact, the type of delivery has not been associated with higher chance of edema attacks. Even so, prophylaxis with C1-INH concentrate was available and infused.7

Table 1 Parameters of hereditary angioedema and thrombophilia determined in the patient.

Parameters Results Reference values
C3 (mg/dL) 87 86–160
C4 (mg/dL) 5 17–45
C1-INH function al (%) 25 >50%
Variant C677T Wild type No mutation
Variant A1298C Homozygous No polymorphism
for A1298C
Homocysteine (µmol/L) 3.5 4.44–13.56

Folic acid was prescribed for its important role in fetal development and the metabolism of homocysteine, which is recognized as an independent risk factor for deep venous thrombosis.10 It is important to note that the MTHFR A1298C mutation is located in the domain that regulates the MTHFR enzyme and is characterized by the alteration of glutamate to alanine, decreasing the enzymatic activity.11 Such a mutation, in both homozygosis (CC) and heterozygosis (AC), has no association with the elevation of plasma homocysteine or the reduction of folic acid in plasma.11,12 Notwithstanding, the analyzed patient presented suggestive events compatible with thrombotic episodes even with normal plasma homocysteine values. Wild type 677CC and homozygous 1298CC genotypes have been found with 0.088 frequency.13

Low-molecular weight heparin, enoxaparin, was introduced to our patient at week 11 of gestation, just after the homozygous mutation of MTHFR was identified. Nevertheless, nadroparin has been reported as an option for the treatment of attacks in patients with HAE.14 However, its efficacy on HAE attacks is still controversial.15,16

Conclusion

In this case, it is relevant to discuss the influence of abdominal attacks as a complicating factor during pregnancy. Although no higher frequency of abortions has been reported in literature, restricted information is available.7 A combined plan of action between obstetricians and specialists is essential to treat HAE.

Funding Source

A grant from the Shire Research Program for Investigators was issued to Dr. Anete S. Grumach (IST-BRA 000778)

REFERENCES

1. Cicardi M, Aberer W, Banerji A, et al. Classification, diagnosis, and approach to treatment for angioedema: Consensus report from the Hereditary Angioedema International Working Group. Allergy. 2014 May;69(5):602–16. 10.1111/all.12380

2. Zuraw BL, Bork K, Binkley KE, et al. Hereditary angioedema with normal C1 inhibitor function: Consensus of an international expert panel. Allergy Asthma Proc. 2012 Nov–Dec;33(Suppl 1):S145–56. 10.2500/aap.2012.33.3627

3. Machado AM, Pires RM, Martins RO, Grumach AS. Pregnancy and Postpartum in hereditary angioedema with C1 inhibitor deficit in women who have no access to therapy. J Investig Allergol Clin Immunol. 2017;27(5):322–3. 10.18176/jiaci.0175

4. Czaller I, Visy B, Csuka D, Fust G, Toth F, Farkas H. The natural history of hereditary angioedema and the impact of treatment with human C1-inhibitor concentrate during pregnancy: A long-term survey. Eur J Obstet Gynecol Reprod Biol. 2010;152:44–9. 10.1016/j.ejogrb.2010.05.008

5. Martinez-Saguer I, Rusicke E, Aygoren-Pursun E, Heller C, Klingebiel T, Kreuz W. Characterization of acute hereditary angioedema attacks during pregnancy and breast-feeding and their treatment with C1 inhibitor concentrate. Am J Obstet Gynecol. 2010;203(131): el–7. 10.1016/j.ajog.2010.03.003

6. Chinniah N, Katelaris CH. Hereditary angioedema and pregnancy. Aust N Z J Obstet Gynaecol. 2009;49:2–5. 10.1111/j.1479-828X.2008.00945.x

7. Caballero T, Farkas H, Bouillet L, et al. International consensus and practical guidelines on the gynecologic and obstetric management of female patients with hereditary angioedema caused by C1 inhibitor deficiency. J Allergy Clin Immunol. 2012;129(2):308–20. 10.1016/j.jaci.2011.11.025

8. Moll S, Varga EA. Homocysteine and MTHFR mutations. Circulation. 2015 Jul 7;132(1):e6–9. 10.1161/CIRCULATIONAHA.114.013311

9. Saguer IM, Ettingshausen CE. Successful management of hereditary angioedema during pregnancy in a patient with heterozygous MTHFR mutation. Ann Allergy Asthma Immunol. 2017;118(6):734–5. 10.1016/j.anai.2017.04.001

10. Deol PS, Barnes TA, Dampier K, John Pasi K, Oppenheimer C, Pavord SR. The effects of folic acid supplements on coagulation status in pregnancy. Br J Haematol. 2004 Oct;127(2):204–8. 10.1111/j.1365-2141.2004.05172.x

11. Van der Put NM, Steegers-Theunissen RP, Frosst P, Trijbels FJ, Eskes TK, Van der Heuvel LP, et al. Molecular genetic analysis in mild hyperhomocysteinemia: A common mutation in the methylenetetrahydrofolate reductase gene is a genetic factor for cardiovascular disease. Am J Hum Genet. 1996;58(1):35–41.

12. Toma TS, Louvison MCP, Bersusa AAS, Bonfim JRA, Prado MF. Low molecular weight heparins for prophylaxis and treatment of deep venous thrombosis in pregnancy. Bol Inst. Saúde (BIS). 2013;14(2):229–36. Available from: http://periodicos.ses.sp.bvs.br/scielo.php?script=sci_arttext&pid=S1518-18122013000200015&lng=e

13. Brown NM, Pratt VM, Buller A, et al. Detection of 677CT/1298AC “double variant” chromosomes: Implications for interpretation of MTHFR genotyping results. Genet Med. 2005;7(4):278–82. 10.1097/01.GIM.0000159904.92850.D5

14. Majluf-Cruz A, Nieto-Martínez S. Long-term follow-up analysis of nadroparin for hereditary angioedema. A preliminary report. Int Immunopharmacol. 2011 Aug;11(8):1127–32. 10.1016/j.intimp.2011.02.007

15. Weiler JM, Quinn SA, Woodworth GG, Brown DD, Layton TA, Maves KK. Does heparin prophylaxis prevent exacerbations of hereditary angioedema? J Allergy Clin Immunol. 2002 Jun;109(6):995–1000. 10.1067/mai.2002.124892

16. Poppelaars F, Damman J, de Vrij EL, et al. New insight into the effects of heparinoids on complement inhibition by C1-inhibitor. Clin Exp Immunol. 2016;184(3):378–88. 10.1111/cei.12777