Evaluation of miR-196a2 expression and Annexin A1 level in children with bronchial asthma Evaluation of miR-196a2 expression and Annexin A1 level in children

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A.A. Ibrahim
A. Ramadan
A.A. Wahby
I.H. Draz
N.R. El Baroudy
T.A. Abdel Hamid


Annexin A1, miR-196a2, Bronchial asthma, Asthmatic children


Background: Annexin A1 (ANXA1) is an important anti-inflammatory mediator that may play a significant role in bronchial asthma. MiR-196a2 can target ANXA1 and therefore may play a role in the pathogenesis of asthma.

Aim of study: This is the first study which aimed to evaluate the expression of miR-196a2 in the serum of asthmatic children and correlate its expression with ANXA1 serum level and asthma severity.

Subjects and methods: The study included 100 asthma patients who were subdivided into three groups (mild, moderate and severe) and 50 healthy control subjects. Assessment of miR-196a2 expression and ANXA1 serum level were done using quantitative reverse transcriptase PCR (RT qPCR) and Elisa techniques, respectively.

Results: Compared to the control group, asthmatic children showed an increased ANXA1 serum level and decreased expression of miR-196a2 (p = 0.001). However, ANXA1 serum level was lower and miR-196a2 expression was higher in severe asthmatic patients compared to moderate asthmatic ones (p = 0.01, 0.03). Pearson’s correlation coefficient revealed no significant correlations between ANXA1 serum level and miR-196a2 expression in the patient group (p = 0.9).

Conclusions: Altered miR-196a2 expression and serum ANXA1 concentration may play a role in the pathogenesis of asthma. In addition, ANXA1 and miR-196a2 may represent potential diagnostic biomarkers for asthma and future targets for therapy.

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