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SHORT COMMUNICATION

Nasal allergen challenge with Blomia tropicalis in children and adolescents

Fausto Yoshio Matsumoto*, Tessa Rachel Tranquilini Gonçalves, Dirceu Solé, Gustavo Falbo Wandalsen

Division of Allergy, Clinical Immunology and Rheumatology, Department of Pediatrics, Universidade Federal de São Paulo, São Paulo, Brazil

Abstract

Background: Local allergic rhinitis (LAR) is a well-defined phenotype in adults, but still there is little data available on children. This scarcity of data can be partly attributed to the lack of standardized protocols for Nasal Allergen Challenges (NAC) in this demographic.

Methods: 20 controls (control group) and 24 patients (rhinitis group) with allergic rhinitis diagnosis sensitized to Blomia tropicalis (Bt) underwent the NAC with Bt. The acoustic rhinometry was performed after instillation of increasing concentrations of Bt (5,000 BU/mL).

Results: The median reduction in the volume of the nasal cavity in its first five centimeters (V5) by the conclusion of the NAC in the rhinitis group was markedly higher than that observed in the control group, at −22.9% (range: −21.1% to −26.2%) compared to −7.7% (ranging: −4.8% to +12.8%).

Conclusions: Our research demonstrates the efficacy and safety of the NAC protocol utilizing Bt in distinguishing allergic from non-allergic children.

Key words: rhinitis, aeroallergens, diagnosis, mites, child, adolescent

*Corresponding author: Fausto Yoshio Matsumoto, Rua do Otonis 725, Vila Clementino. Email address: [email protected]

Received 15 August 2024; Accepted 28 Sepember 2024; Available online 1 November 2024

DOI: 10.15586/aei.v52i6.1193

Copyright: Matsumoto FY, et al.
License: This open access article is licensed under Creative Commons Attribution 4.0 International (CC BY 4.0). http://creativecommons.org/licenses/by/4.0/

Introduction

Local allergic rhinitis (LAR) is a localized nasal allergic response in the absence of systemic atopy characterized by the local production of specific IgE (sIgE) antibodies.1 While LAR is a well-defined phenotype in adults, however, there is little data available on children. This scarcity of data can be partly attributed to the lack of standardized protocols for Nasal Allergen Challenges (NACs) in this demographic.

Blomia tropicalis (Bt) is a mite commonly linked with early sensitization and ongoing symptoms of asthma and allergic rhinoconjunctivitis in countries with tropical and subtropical climates, where high humidity and temperature promote its proliferation.2 Molecular analysis reveals that approximately 64% of the allergens found in Bt are unique,3 and this distinctiveness complicates the use of standardized testing protocols designed for other mites and underscores the need for a tailored approach. Bt has been tested in NAC4,5 in adults, but there are no validated protocols in pediatric population.

Methods

We developed a standardized NAC protocol with Bt for patients aged between 7 and 18 years. Participants were categorized into two groups: a) a control group without symptoms and/or diagnosis of allergic respiratory conditions and a negative immediate hypersensitivity skin test for a panel of aeroallergens (D. pteronyssinus, D. farinae, Bt, cockroach, dog, cat, and fungi); b) a group consisting of patients with rhinitis (according to the ARIA initiative)6 who had a positive skin test for Bt (mean papule diameter of ≥ 3 mm). The study was approved by the Institutional Research Ethics Committee. Written informed consent was obtained from all legal guardians and individual participants included in the study.

During the NAC, Bt extracts (FDA ALLERGENIC® - Brazil, 5,000 UBE/ml, 0.15 mcg/mL Blo t 5) were diluted at different levels in 0.9% saline solution. Objective monitoring was conducted using acoustic rhinometry (AcR) (A1, GM Instruments, Scotland - UK) alongside subjective evaluations through a symptom questionnaire. These assessments were consistently performed in triplicate by the same observer, adhering to international recommendations.7 Baseline metrics were established following the administration of 0.15 mL of saline solution. Subsequently, 0.15 mL of Bt solution, at progressively increasing concentrations, was sprayed into each nostril at 10-min intervals (Figure 1). After each administration, new AcR measurements and symptom questionnaire (Table 1) assessments were taken.

Figure 1 Flowchart used to perform the NAC with Bt (5,000 UBE/mL) in children and adolescents.

Table 1 Symptom questionnaire registered during specific NPT with Blomia tropicalis.

Symptom Points
Nasal secretion at anterior rhinoscopy (examiner’s judgment) As before/normal 0
Slight increase/minor amounts visible 1
Pronounced 2
Irritation 0–2 sneezes 0
3–5 sneezes 1
> 5 sneezes 2
Distant symptoms None 0
Watery eyes and/or
Palatal itching and/or
Deep aural itching
1
Conjunctivitis and/or
Chemosis and/or
Urticaria and/or
Cough and/or
Dyspnea
2

The NAC was concluded upon administering the highest concentration of Bt, on observing a reduction in the volume of the nasal cavity in its first 5 cm (V5 cm3) by ≥20%, or when a score of >3 was recorded on the symptom questionnaire, with parameters previously used in standardizations in children and adolescents.8,9 Additionally, the two smallest cross-sectional areas (MCA1 and MCA2 cm2) in each nostril were measured.

The clinical and demographic profiles of the participating children are detailed in Table 2.

Table 2 Demographic and clinical characteristics observed in rhinitis and control groups.

Rhinitis group (N = 24) Control group (N = 20) P
Age (months)* 144 (124–188) 120 (86–149) 0.02
Male gender (%) 66.7 35.0 0.04
Asthma (%) 45.8 0 <0.001
Atopic dermatitis (%) 25.0 15.0 0.33
TNSS* 6 (5–8) 2 (0–3) <0.001
MCA1 (cm2)* 1.03 (0.78–1.32) 1.19 (0.88–1.28) 0.65
MCA2 (cm2)* 1.91 (1.03–2.18) 2.23 (1.73–2.59) 0.04
V5 (cm3)* 9.18 (7.19–11.59) 9.51 (7.99–10.66) 0.62

*averages and interquartile ranges.

MCA1 and MCA2: The two smaller cross-sectional areas; TNSS: total nasal symptom score; V5: Volume of the first 5 cm of the nasal cavity.

Results

In the rhinitis group, 22 out of 24 patients (91.7%) showed positive results to NAC, all registering significant changes in AcR parameters. Only one patient showed a concurrent shift in their symptom score. We observed a higher incidence of positive NAC outcomes until the 1:100 dilution concentration in 18 out of the 22 positive cases, while a smaller fraction, 4 out of 22, demonstrated positive reactions at the 1:10 dilution.

The median drop in V5 by the end of the NAC in the rhinitis group was markedly higher than that observed in the control group at −22.9% (range: −21.1−−26.2%) compared to −7.7% (range: −4.8−+12.8%), depicted in Figure 2A (P < 0.001). This was also observed in the questionnaire, depicted in Figure 2B (P = 0.006).

Figure 2 (A) The percentage reduction in the volume of the first 5 cm of the nasal cavity (V5) at the conclusion of the NAC with Bt for both the allergic rhinitis group (in red) and the control group (in green). (B) Final questionnaire symptom score values at the conclusion of the NAC with Bt in the allergic rhinitis group (red) and the control group (green).

No individual in the control group registered a positive NAC outcome, and no participants experienced late reactions, and adverse nasal or extranasal effects during or after NAC.

Discussion

This study has some limitations such as the small number of participants and the lack of data on the collection of nasal-specific IgE or other cytokines that could further substantiate the detection of a localized allergic response. However, recently published documentation for standardizing the NAC in adults10,11 does not require such analyses.

NAC is a relatively simple and safe procedure that has been used for many years as a research tool to investigate the pathophysiology of rhinitis. It has clinical utility in certain clinical settings and is suitable as an office-based setup to diagnose LAR. NAC can be standardized to a good extent, but the results (as a subjective nasal symptom score and/or an objective nasal air flow rate) are sometimes difficult to interpret, especially in the pediatric age group.12 Despite that, NAC continues to be the gold standard for diagnosing LAR.

In conclusion, our research demonstrates the efficacy and safety of the NAC protocol utilizing Bt in distinguishing allergic children from nonallergic. This protocol not only holds the potential for broadening the spectrum of allergens used in nasal provocation tests but also enhances the diagnostic precision of LAR in pediatric patients.

REFERENCES

1 Rondón C, Fernandez J, Canto G, Blanca M. Local allergic rhinitis: Concept, clinical manifestations, and diagnostic approach. J Investig Allergol Clin Immunol. 2010;20(5):364–71.

2 Caraballo L, Zakzuk J, Lee BW, Acevedo N, Soh JY, Sánchez-Borges M, et al. Particularities of allergy in the Tropics. World Allergy Organ J. 2016;9(1):20. 10.1186/s40413-016-0110-7

3 Arruda LK, Rizzo MC, Chapman MD, Fernandez-Caldas E, Baggio D, Platts-Mills TAE, et al. Exposure and sensitization to dust mite allergens among asthmatic children in São Paulo, Brazil. Clin Exp Allergy. 1991;21(4):433–9. 10.1111/j.1365-2222.1991.tb01683.x

4 Stanaland BE, Fernández-Caldas E, Jacinto CM, Trudeau WL, Lockey RF. Positive nasal challenge responses to Blomia tropicalis. J Allergy Clin Immunol. 1996;97(5):1045–9. 10.1016/s0091-6749(96)70256-7

5 Wang DY, Goh DYT, Ho AKL, Chew FT, Yeoh KH, Lee BW. The upper and lower airway responses to nasal challenge with house-dust mite Blomia tropicalis. Allergy. 2003;58(1):78–82. 10.1034/j.1398-9995.2003.23746.x

6 Bousquet J, Khaltaev N, Cruz AA, Denburg J, Fokkens WJ, Togias A, et al. Allergic Rhinitis and its Impact on Asthma (ARIA) 2008 update (in collaboration with the World Health Organization, GA2LEN and AllerGen). Allergy. 2008;63(Suppl 86):8–160. 10.1111/j.1398-9995.2007.01620.x

7 Hilberg O, Pedersen OF. Acoustic rhinometry: Recommendations for technical specifications and standard operating procedures. Rhinol Suppl. 2000;16:3–17.

8 Wandalsen GF, Mendes AI, Matsumoto F, Solé D. Acoustic rhinometry in nasal provocation tests in children and adolescents. J Investig Allergol Clin Immunol. 2016;26(3):156–60. 10.18176/jiaci.0036

9 Matsumoto FY, Gonçalves TRT, Solé D, Wandalsen GF. Specific nasal provocation test with Dermatophagoides pteronyssinus, monitored by acoustic rhinometry, in children with rhinitis. Am J Rhinol Allergy. 2017;31(1):7–11. 10.2500/ajra.2017.31.4392

10 Augé J, Vent J, Agache I, Airaksinen L, Campo Mozo P, Chaker A, et al. EAACI Position paper on the standardization of nasal allergen challenges. Allergy. 2018;73(8):1597–608. 10.1111/all.13416

11 Cho SH, Nanda A, Keswani A, Adinoff A, Baroody FM, Bernstein JA, et al. Nasal allergen challenge (NAC): Practical aspects and applications from an EU/US perspective—A Work Group Report of the AAAAI Rhinitis, Rhinosinusitis and Ocular Allergy Committee. J Allergy Clin Immunol. 2023;151(5):1215–22. 10.1016/j.jaci.2023.02.014

12 Hossenbaccus L, Ellis AK. Expert review of clinical immunology the use of nasal allergen vs allergen exposure chambers to evaluate allergen immunotherapy. Expert Rev Clin Immunol. 2021;17(5):461–70. 10.1080/1744666X.2021.1905523